The Southwest Florida Expressway Authority is an 8-person body including transportation officials from Lee and Collier Counties, the role of which is to raise toll revenue to widen and improve major arteries throughout both counties. Of particular priority is the needed revenue to widen the I-75 freeway beyond the 6-lane expansion due to begin in March 2007.

  Interstate 75 is the county's only fully-controlled-access freeway, and has nine interchanges within Lee County, linking the area to Naples, Fort Lauderdale and Miami to the south and east; and Sarasota and Tampa to the north.

U.S. Highway 41 runs the length of Lee County, and is the county's main north-south arterial highway. It is a major commercial corridor, running as an elevated highway through the center of downtown Fort Myers, continuing south as a multi-lane divided surface highway through the communities of South Fort Myers, San Carlos Park, Estero, and Bonita Springs. From north-to-south, the highway's name starts as "North Tamiami Trail", changes to "Cleveland Avenue" through the incorporated limits of the City of Fort Myers, then changes to "South Tamiami Trail until the border with Collier County.

State Road 80 's western terminus is in downtown Fort Myers. The multi-lane highway runs east-northeast along the southern banks of the Caloosahatchee River as "Palm Beach Boulevard" within the county, traversing the state of Florida to connect the area with LaBelle, Clewiston, and West Palm Beach.

State Road 82 's western terminus is in downtown Fort Myers. The highway is called "Dr. Martin Luther King Jr. Boulevard" within the incorporated limits of the City of Fort Myers, becoming "Immokalee Road" as it passes through Lehigh Acres and connects the area to Immokalee.

State Road 884 is Lee County's main east-west arterial highway. Its western terminus is in the incorporated limits of the City of Cape Coral. Within Cape Coral, the highway is named "Veterans' Memorial Parkway", and is a multi-lane controlled access highway. The road crosses the Caloosahatchee River as an elevated highway across a toll bridge, interchanging with U.S. Highway 41, then becomes a multi-lane divided surface highway through Lehigh Acres.

Major Road Bridges

Caloosahatchee River Bridge (U.S. Highway 41): 4-travel-lane single-span bridge connects North Fort Myers with Fort Myers, over the Caloosahatchee River.

Cape Coral Bridge (College Parkway/Cape Coral Parkway): Two 2-travel-lane spans (one eastbound, one westbound) connect Cape Coral with South Fort Myers, over the Caloosahatchee River.

• Edison Bridge (State Road 739): Two 3-travel-lane spans (one northbound, one southbound) over the Caloosahatchee River.
• State Road 31: 2-travel-lane single-span bridge over the Caloosahatchee River.
• Interstate 75: Two 3-travel-lane spans over the Caloosahatchee River.
• Matanzas Pass Bridge (State Road 865): 3-travel-lane single-span bridge connecting the mainland to the barrier islands.
• Midpoint Memorial Bridge (State Road 884): Two 2-travel-lane spans (one eastbound, one westbound) connect Cape Coral with Fort Myers
• Sanibel Causeway (State Road 867): series of 2-travel-lane single-span bridges and two 3-travel-lane island causeways crossing the mouth of the Caloosahatchee River at the Gulf of Mexico, including a drawbridge over the Intracoastal Waterway. The causeway connects South Fort Myers with Sanibel.

Mass Transportation

Fixed-route bus service is provided by the Lee County Transit Department, operated as "LeeTran". Several routes extend outward from the Downtown Intermodal Transfer Center; in addition, suburb-to-suburb routes are operated, as well as park-and-ride service to and from both Fort Myers Beach and Southwest Florida International Airport. The Downtown Intermodal Transfer Center also serves as an intermediate stop on Greyhound Lines bus service.

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